The Australian soap - which first aired on BBC One in the UK in 1986 - is set to come to an end this Summer.
Last month, it was reported that Channel 5 producers spent weeks in talks with producers at Fremantle Australia to strike a deal to keep the series on air, but those talks have reportedly failed.
Channel 5 said in a statement: "Neighbours will no longer air on Channel 5 beyond this summer. It has been a much-loved part of our schedule for more than a decade, and we'd like to thank the cast, Fremantle and all of the production team for their fantastic work on this iconic series...
"We'd also of course like to thank the fans for their loyal support of Neighbours across the years. We recognise that there will be disappointment about this decision, however our current focus is on increasing our investment in original UK drama, which has strong appeal for our viewers."
A Network 10 spokesperson said: "As outlined in the email to Neighbours cast and crew, it is our intention to continue our association with Neighbours if another broadcast partner comes forward..."
It's now been revealed, exclusively by The Sun, that no deal has been struck to keep the show on air, meaning the show will end for good this Summer.
For now, Neighbours airs weeknights at 6pm on Channel 5.